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Abstract
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Background and Objective: This research is purported to evaluate the antimicrobial effect of silver nanoparticles on the gram-positive and gram-negative bacteria that are produced to be utilized in preparing silver nanocomposite with the biodegradable antibacterial property through the solution method. The current study aimed to produce antimicrobial biodegradable silver nanocomposites for food coating production and impact on a wide range of bacteria. Material and Methods: In this research, to evaluate the antibacterial properties of silver nano-particles, several steps have been applied. First, they were synthesized through a chemical reduction method using NaBH4 and it was analyzed by x radiation diffraction, UV-VIS, DLS and SEM nanometric tests. In the next step, Staphylococcus aureus and Escherichia coli were used as positive and negative gram bacteria indicators, simultaneously. Afterward, the MIC, MBC, and Inhibition Zone levels were measured. Finally, nanocomposite was produced during the solution blending method, and its antibacterial property was assessed using the inhibition zone method. Results and Conclusion: The obtained results indicated that silver nanoparticles with 20 and 50 micrograms in Liter concentrations have inhibitory effects, respectively, on Staphylococcus aureus and Escherichia coli. Also, the concentration of 40 to 60 mg.l-1 has a lethal effect on the mentioned bacteria again, respectively. According to the results, the highest antibacterial effect is observed on the gram-positive Staphylococcus aureus bacteria. Despite this, in the inhibition zone tests, a 3-5mm zone was observable around the silver nanoparticles disc in the cultures of the aforementioned microorganisms. In the inhibition zone test of the produced nanocomposites too, the appearing zone was expected regarding the amounts. Test results were calculated in three repetitions, and the value estimated through ANOVA was significant at P<.0001. It was concluded that silver nanoparticles are useful in positive gram and gram-negative bacteria in terms of inhibition and destruction. Moreover, it was proven that using the mentioned method has a great influence on the antibacterial properties of nanocomposite. Conflict of interest: We hereby certify that there is not any actual or potential conflict of interest.
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